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exflagellation medium  (Thermo Fisher)


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    Structured Review

    Thermo Fisher exflagellation medium
    Exflagellation Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/exflagellation+medium/HEPES/pmc12003780-53-10-27
    Average 99 stars, based on 1 article reviews
    exflagellation medium - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: Antimalarial Iron Chelator FBS0701 Blocks Transmission by Plasmodium falciparum Gametocyte Activation Inhibition
    Article Snippet: .. Eleven microliters of 10× exflagellation medium (RPMI 1640, 200 mM HEPES, 40 mM sodium bicarbonate, 100 mM glucose [pH 8]) was added, and the gametocytes were incubated at room temperature for 30 min. Next, 11 μl of 10× CyQUANT (background suppressor permeable to dead cells; Life Technologies, Grand Island, NY, USA) and SYBR green I (dye for the detection of live cells; Life Technologies, Eugene, OR, USA) diluted in 1× PBS were added, and the gametocytes were incubated at room temperature for 1 h. Fluorescence was determined at 535 nm following an excitation at 485 nm in a plate reader (HTS7000; PerkinElmer). ..

    CyQUANT Assay:

    Article Title: Antimalarial Iron Chelator FBS0701 Blocks Transmission by Plasmodium falciparum Gametocyte Activation Inhibition
    Article Snippet: .. Eleven microliters of 10× exflagellation medium (RPMI 1640, 200 mM HEPES, 40 mM sodium bicarbonate, 100 mM glucose [pH 8]) was added, and the gametocytes were incubated at room temperature for 30 min. Next, 11 μl of 10× CyQUANT (background suppressor permeable to dead cells; Life Technologies, Grand Island, NY, USA) and SYBR green I (dye for the detection of live cells; Life Technologies, Eugene, OR, USA) diluted in 1× PBS were added, and the gametocytes were incubated at room temperature for 1 h. Fluorescence was determined at 535 nm following an excitation at 485 nm in a plate reader (HTS7000; PerkinElmer). ..

    SYBR Green Assay:

    Article Title: Antimalarial Iron Chelator FBS0701 Blocks Transmission by Plasmodium falciparum Gametocyte Activation Inhibition
    Article Snippet: .. Eleven microliters of 10× exflagellation medium (RPMI 1640, 200 mM HEPES, 40 mM sodium bicarbonate, 100 mM glucose [pH 8]) was added, and the gametocytes were incubated at room temperature for 30 min. Next, 11 μl of 10× CyQUANT (background suppressor permeable to dead cells; Life Technologies, Grand Island, NY, USA) and SYBR green I (dye for the detection of live cells; Life Technologies, Eugene, OR, USA) diluted in 1× PBS were added, and the gametocytes were incubated at room temperature for 1 h. Fluorescence was determined at 535 nm following an excitation at 485 nm in a plate reader (HTS7000; PerkinElmer). ..

    Fluorescence:

    Article Title: Antimalarial Iron Chelator FBS0701 Blocks Transmission by Plasmodium falciparum Gametocyte Activation Inhibition
    Article Snippet: .. Eleven microliters of 10× exflagellation medium (RPMI 1640, 200 mM HEPES, 40 mM sodium bicarbonate, 100 mM glucose [pH 8]) was added, and the gametocytes were incubated at room temperature for 30 min. Next, 11 μl of 10× CyQUANT (background suppressor permeable to dead cells; Life Technologies, Grand Island, NY, USA) and SYBR green I (dye for the detection of live cells; Life Technologies, Eugene, OR, USA) diluted in 1× PBS were added, and the gametocytes were incubated at room temperature for 1 h. Fluorescence was determined at 535 nm following an excitation at 485 nm in a plate reader (HTS7000; PerkinElmer). ..



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